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OBJECTIVE To evaluate the pharmacological actions of the Formulae of Qinwenbaiduyin and Huanglianjiedutang in antibacterial experiment in vitro. METHODS Test tube method and plating method were employed in antibacterial experiment while test was conducted in antiviral experiment with extract of the Formula by water and then observing the pathological changes in the lungs after medication. RESULTS The minimal inhibirory concentration (MIC) effect of Qinwenbaiduyin was 15.6 mg/ml, the minimal bacteriocidal concentration (MBC) was 31.2mg/ml to Staphylococcus aureus; minimal inhibirory concentration (MIC) effect was 7.6mg/ml, MBC was 15.6mg/ml to Escherichia coli. The MIC effect of Qinwenbaiduyin was 15.6 mg/ml, the MBC was 31.2mg/ml to S. aureus and E. coli. CONCLUSIONS The Formulae of Qinwenbaiduyin and Huanglianjiedutang have antibacterial effect on S. aureus and E. coli in experiment.
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Objective To explore the nervous mechanism of intestinal function disorder after intestine congestion through observing the changes of acetylcholinesterase (AchE) neuron and nitric oxide synthase neuron (NOS) expression in nervous system of rat intestinal tract. Methods Sprague Dawley rats were divided into experimental groups (intestine congestion 20min and 60min group) and the control group. Then the spreading specimens of intestinal myen-teric plexus of ileums were collected and stained by AchE and nicotinamide-adenine dinucleotide phosphate-D (NADPH-d) histochemistry, to observe and compare the density of distribution and staining of AchE and NOS positive neurons. Result Compared to the control group, the number and positive expression of AchE positive neuron in intestinal myen-teric plexus of ileums in the experimental group rats decreased (P
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Alopeeia areata (AA) has been well recognized with familial tendencies, but the genetic basis of this clinical observation remains unknown. The cytokine interleukin-1 receptor antagonist (ILlra) is a potent anti-inflammatory protein that can prevent immune-mediated inflammatory response in the skin. We characterized a polymorphism within the gene for this cytokine ILlra in this study and tested the gene as a possible marker in patients with alopecia areata. We have determined allele frequencies of the polymorphic cytokine genes in a control population and a group of 72 patients with alopecia areata. The frequency of allele 2 of interleukin-1 receptor antagonist in patients with AA was significantly higher than that of control group. It suggests that interleukin-1 receptor antagonist gene may be a candidate gene or severity factor for alopecia areata.
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Objective To observe the toxic effects of mercuric chloride (HgCl2) on the productive function of male mice with lower dosage exposune. Methods 4 week-aged male ICR mice were randomly divided into 3 exposure groups, and control group. The 3 exposure groups were treated with doses of 0.25, 0.50, 1.00 mg/kg HgCl2 by peritoneal injection respectively, one time per 3 days, 10 times in total. After exposure to HgCl2 for 50 days, the male mice were mated with female mice non-exposed to HgCl2 in a ratio 1∶2. The pregnant rate, number of pups whelped per group, body weight of offspring, testis index, sperm count, sperm motility rate, abnormal sperm rate were observed. Results The pregnant rates were 100%, 100%, 83.33% and 66.67% for control group, 0.25 mg/kg group, 0.50 mg/kg group and 1.00 mg/kg group respectively during 1-week conception, 100%, 100%, 83.33% and 75% for above corresponding groups respectively during 3-weeks conception respectively. The pregnant rate of 1.00 mg/kg group was significantly lower than that of control during 1-week conception (P
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Objective To observe the effects of CO2 pneumoperitoneum on the enteric motor function and acetylcholine esterase(AchE) neuron and nitric oxide synthase(NOS) neuron in the enteric nervous system,and explore the neuromechanism of the CO2 pneumoperitoneum on renteric motor function.Methods Thirty-six Sprague Dawley rats were randomly divided into experiment group(n=24) and control group(n=12).The experiment group was divided into two subgroups namely pneumoperitoneum 30min group and pneumoperitoneum 60min group(12 each) based on the maintenance time of pneumoperitoneum.Rats in each group were gavaged with medicinal carbon powder,and then the transmission of carbon powder in small intestine was determined.The spreading specimens of intestinal myenteric plexus of small intestine were prepared and the stained AchE and NOS neurons were observed and compared.Results The propellant velocity of carbon powder was slower in pneumoperitoneum 60min group than that in pneumoperitoneum 30min group and control group(28.55%?3.45% vs 45.90%?6.30%,48.25%?5.28%,P0.05).The number of positive expression of AchE neurons in intestinal myenteric plexus decreased in pneumoperitoneum 60min group compared with that in pneumoperitoneum 30min group and control group(48.00?3.16 vs 58.82?4.62,61.83?4.17,P0.05).The number of positive expression of NOS neurons in intestinal myenteric plexus increased in pneumoperitoneum 60min group compared with that in pneumoperitoneum 30min group and control group(42.17?4.45 vs 32.50?4.34,30.83?3.6,P0.05).Conclusions Prolonged CO2 pneumoperitoneum can affect or damage cholinergic neurons and nitroxidergic neurons in the enteric nervous system to some extent,and it may be the underlying mechanism of the intestinal motor dysfunction after operation.
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Objective To observe the changes in enteric motor function and acetylcholine esterase(AchE)neuron and nitric oxide synthase neuron(NOS)in enteric nervous system after different abdominal operations,and to explore the mechanism of these changes.Methods Sprague Dawley rats were divided into experimental groups(laparotomy group,intestine massage group and intestinal operation group)and the control group.Rats in each group were gavaged with medicinal carbon powder,and then the transimission of carbon powder in small intestine was determined,and the spreading specimens of intestinal myenteric plexus of small intestine and large intestine were collected and stained with AchE and nicotinamide-adenine dinucleotide phosphate-D(NADPH-d)for histological study,and the density of distribution and positive stained AchE and NOS neurons were observed and compared.Results The changes in enteric motor function:the propellant rate of carbon powder was slower in enteric massage group and intestinal operation group than that in laparotomy group and control group(18.1?2.8,25.7?3.5 vs 47.4?3.6,49.8?4.5,P0.05).Compared with the control group,the number and positive expression of AchE positive neuron in intestinal myenteric plexus decreased in small intestine and large intestine in intestinal operation group and intestine massage group(small intestine 43.0?3.0,52.2?3.3 vs 65.3?3.9,P